产品索引 | 5871 |
中文名称: | 植物基因组DNA提取试剂盒 |
英文名称: | MagExtractor®-Plant Genome- |
产品编号: | NPK- 500 |
产品类别: |
分子生物学 |
生产厂家: | TOYOBO |
产品价格: | 800元 |
产品规格: | 50次 |
Nucleic Acid Purification Kit
MagExtractor
-Plant Genome-
使用说明书
(Code No.:NPK- 500)
TOYOBO CO., LTD. Biochemical Operations Department
OSAKAJAPAN
—目录—
目录 页数
1.前言.......................................................................................................................... 1
2.使用前须知.............................................................................................................. 1
3.试剂盒中所包含的物品.......................................................................................... 2
4.操作程序.................................................................................................................. 2
[1]试剂盒以外所需要的其他物品...................................................................... 2
(1)试剂............................................................................................................. 2
(2)器具及器材................................................................................................. 3
[2]抽提流程.......................................................................................................... 3
[3]样品前处理...................................................................................................... 4
[4]使用MFX-2000进行Plant DNA抽提......................................................... 4
(1)操作程序的选择......................................................................................... 4
(2)加温block以及回收block温度的设定.................................................. 4
(3)附带专用过滤器的tip安装....................................................................... 5
(4)专用试管的安装......................................................................................... 5
(5)试剂的安装................................................................................................. 6
(6)样品的安装................................................................................................. 8
(7)抽提开始..................................................................................................... 8
(8)样品的回收................................................................................................. 8
[5]使用手工方法进行Plant DNA抽提............................................................. 8
5.一般样品的分析...................................................................................................... 9
[1]DNA定量测定................................................................................................ 9
[2]电泳............................................................................................................... 10
[3]PCR............................................................................................................... 10
6.抽提实例................................................................................................................ 10
7.疑难解答................................................................................................................ 11
注意事项
本试剂盒中所包含的所有试剂属研究用试剂。请不要用于诊断或临床等场合。在使用本试剂盒的时候,请严格遵守常用的实验室安全操作程序。
Hoffman-La Roche Ltd.拥有PCR方法的专利权。